MY TIPs

A method for C-banding



1. A Lazy Method to Isolate High Molecular Weight Genomic DNA from Solid Tissues step by step
2. Easy Loading to Agarose Gel with 4-Channel Pipette
3. Tips for MW Marker and Loading Dye
4. Pitfall in Dephosphorilation of Vector
5. Reducing PCR-Cloning Artifacts by Single Re-Extension
6. A Hasty Method to Prepare Competent E. coli step by step
7. Colony Direct PCR to Skip Mini-Prep for Sequencing
8. Tips for PCR Direct Sequencing
9. Using ET terminator kit on ABI3100 (Win2000 version)
10. Economy Sequencing Protocols for ABI3100
11. Using phred/phrap/consed
12. Using BioEdit
13. How to Install Auto-primer   (for Linux)  (for Windows)
14. Fontana di Trivia
15. Useful Small Files
16. Tips for ABI373

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These technical tips are based on my personal experiences and may be different from suppliers' recommendations. These technical tips may not work in some other laboratory systems.


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